Antibody Techniques by Vedpal S. Malik, Erik P. Lillehoj PDF

By Vedpal S. Malik, Erik P. Lillehoj

ISBN-10: 0124664601

ISBN-13: 9780124664609

The applicability of immunotechniques to a large choice of analysis difficulties in lots of components of biology and chemistry has increased dramatically during the last 20 years ever because the advent of monoclonal antibodies and complicated immunosorbent thoughts. Exquisitely particular antibody molecules supply technique of separation, quantitative and qualitative research, and localization important to an individual doing organic or biochemical research.
This useful advisor to immunotechniques is principally designed to be simply understood by way of individuals with little functional event utilizing antibodies. It basically provides distinct, easy-to-follow, step by step equipment for the generally used strategies that take advantage of the original homes of antibodies and should support researchers use antibodies to their greatest virtue.

Key Features
* targeted, easy-to-follow, step by step protocols
* handy, easy-to-use format
* large sensible information
* crucial historical past information
* invaluable tricks

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1981). IgG antibodies to phosphorylcholine exhibit more diversity than their IgM counterparts. Nature (London) 291, 2 9 - 3 4 . George, J . , Penner, S. J . , Weber, J . , Berry, J . , and Claflin, J. L. (1993). Influence of membrane Ig receptor density and affinity on B cell signaling by antigen. /. Immunol. 151, 5955-5965. Germain, R. N. (1994). MHC-dependent antigen processing and peptide presentation: Providing ligands for T lymphocyte activation. Cell 76, 2 8 7 - 2 9 9 . Gershon, R. , and Kondo, K.

Rabbitts, T. , and Bentley, D. L. (1980). The role of gene deletion in the immunoglobulin heavy chain switch. Nature (London) 283, 3 5 1 - 3 5 6 . Reinherz, E. , and Schlossman, S. (1979). Separation of functional subsets of T cells by monoclonal antibody. Proc. Natl. Acad. Sci. USA 76, 4061-4065. Rogers, J . , and Wall, R. (1980). Two mRNA's with different 3' ends encode membrane-bound and secreted forms of immunoglobulin JJL chain. Cell 20, 3 0 3 - 3 1 2 . , and Alt, F. W. (1988). Mitogen plus interleukin-4 induction of Ce transcription in B lymphoid cells.

Bothwell, A. , and Baltimore, D. (1980). Synthesis of secreted and membrane-bound immunoglobulin /JL heavy chains is directed by mRNAs that differ at their 3' ends. Cell 20, 2 9 3 - 3 0 1 . Alt, F. , Oltz, E. , Young, F . , Groman, J . , and Chen, J. (1992). Immunol. Today 13, 3 0 6 - 3 1 4 . Amzel, L. , and Poljak, R. J. (1979). Three-dimensional structure of immunoglobulins. Annu. Rev. Biochem. 48, 961. , Li, X . , Bajorath, J . , Grosmaire, L. , Roberts, R. , Noelle, 44 Ian M. Zitron R. J .

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Antibody Techniques by Vedpal S. Malik, Erik P. Lillehoj


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